Table 3.

Primers used for complete genome sequence amplification of strain GD

Primera	Location (bp)	Upstream primer	Downstream primer	Length (bp)
1	22–1571	TATATATCTATTGCACTAGCC	AGTCAGACAGACAACACGCT	1549
2	1025–1677	GCAGACTTGTTGGTGAGGTTA	CACAAGTTCCGAAACACTAAA	652
3	1530–2687	TGGAGGGACATCTTTGCTAT	TCTGTCTCAACTTCAATGGG	1157
4	2566–3961	TGGTGAAACTACTGTGAAGG	CACACCATCTACAAGAACAT	1395
5	3642–5168	TGTTAACGCCGCAAATGAG	GGCAACTTGGAATCTTCCT	1527
6	4873–6504	TAAAAAGAGTAAGAGCAAGA	AGGAGACATAAGTGTATTTTG	1631
7	6534–7920	CGTCTACACTAACTCAGGCTA	CCTGACTCCACTAGGTTGAA	1386
8	7890–9260	GAAATTGTTGGTTACACCCAC	TAGAACGCATAGTAACGGGG	1370
9	9247–10664	TCAGTAGGCGTTTGAAAGG	ATAGGCAACACACGGTCA	1417
10	10594–12110	GGCATAGGTTGGATGTTTACT	CACAGAAGCCCCTCCGTAA	1516
11	11954–12523	GTCTTACAGTCTAAAGGAT	AGCACAGTTACGCTTCAAAT	569
12	12418–13418	ACTTAGACAACCAAAACCCTT	TCATAATAACACCGAGTTCCT	1000
13	13261–13978	CCCTCCTCAAGTATGATTAT	ATAGTGGGCAGGACATTCTT	717
14	13865–15044	TATTTTGTTTAGAGGTGACG	AGGAATAGTCAATAAGCAT	1179
15	14712–16138	CTGATTCTAAGTGTTGGGTT	TCCTTTGAGGTACTATGCGA	1427
16	15516–16465	ACTCGCTAAGACGCTTTGCT	TTAGGACAACGGTAACACTTC	950
17	16283–17509	GTGACATTCTATTGGTTGAT	ACAGACTTGTTCCTTGCCT	1226
18	17503–19024	TCACTTGAGAGCTTTGT	TAAACATACAGATTCGCT	1521
19	18628–19634	ATCAAACAACTCTGCCTACA	CCACATTCATCATAATACCACT	1007
20	18945–20442	AAGCGGTATYCNTATGTAGA	ATAGTRCAVACAAAAKRGTCA	1498
21	20189–20578	TTATTTGGGTGACAGTGG	ACCACTAATAATACCAACAG	389
22	20437–22232	AAGGTTAATCCCTGTGAAG	AGTYTCVGTAAGAATAGCA	1795
23	21873–22919	AAGGTTAATCCCTGTGAAG	AGTYTCVGTAAGAATAGCA	1047
24	22723–23837	CTTTTGCHACTCAGATDCA	AGATTTCTTACCACACTTACT	1115
25	23791–24992	AGGGGCTTAATGACTCTCTT	CTGACCTTCACAATAAAGAAC	1201
26	24796–25894	GCAGCGATAATACTTACAGT	TCTGCTTGTCCTGCTTTGT	1099
27	25121–26453	GTGACCGAAGCGGAAATAA	TCAGAGGAATGAAGTCCCAAC	1333
28	26891–27706	GGTGATTCTCAAGATGGTAT	GCTCTAACTCTATACTAGCCT	1416

^aPrimer locations are indicated according to strain TW2575/98 (DQ646405)