Fig. 2.
Two pin tool alignment frames used in the procedure. The A frame has four holes for guide pins that allow a pin tool loaded with 6 rows of 48 pins to transfer the peptide library into one of four quadrants of a 1536-well plate (see Fig. 1). The B frame has 32 pairs of holes to allow a 48 pin strip to transfer kinase/ATP to each row of a 1536-well plate.