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. Author manuscript; available in PMC: 2016 Mar 9.
Published in final edited form as: FEBS Lett. 2010 Oct 29;584(23):4761–4768. doi: 10.1016/j.febslet.2010.10.055

Fig. 2.

Fig. 2

Biochemical evidence for the biosynthesis of menaquinone in intraerythrocytic stages of P. falciparum. (A) Profile of RP-HPLC analysis (C18 column, Protocol I) of P. falciparum extracts [17] from ring, trophozoite and schizont stages (1 × 109 parasites per stage) metabolically labelled with [1-(n)-3H]GGPP. UNIPOINT™ Software (Gilson Inc.) was used as the operational and analytical system. Samples were co-injected with MQ-4 standard. (B) The radioactive peaks corresponding to the retention time of UQ and MQ-4 were plotted from extracts (according to Bekker et al. [18]) of 1 × 109 schizont stages metabolically labelled with [1-(n)-3H]GGPP, followed by RP-HPLC analysis (Protocol II). Sample was co-injected with UQ and MQ-4 standards. MQ-4: Menaquinone; UQ: ubiquinone.