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. Author manuscript; available in PMC: 2016 Mar 9.
Published in final edited form as: FEBS Lett. 2010 Oct 29;584(23):4761–4768. doi: 10.1016/j.febslet.2010.10.055

Fig. 4.

Fig. 4

Menaquinone and Ubiquinone levels change in response to Ro 48-8071 treatment. (A) Ro 48-8071 inhibited parasite growth in a concentration-dependent manner (IC50 = 4.5 ± 0.3 μM; mean ± S.D., n = 3 experiments), calculated according to Moneriz et al. [23]. (B) Decrease of MQ-4 contents in the schizont stages after the treatment with 4.5 μM of Ro 48-8071. The effect of Ro 48-8071 on MQ biosynthesis was analyzed (by RP-HPLC, Protocol II, with the extraction methodology described by Bekker et al. [18]) as the relationship between control and Ro 48-8071 treated parasites (2.7 × 109) metabolically labelled with [1-(n)-3H]GGPP (mean ± S.D., n = 3 experiments). Significant differences comparing control parasites and parasites treated with Ro 48-8071 (One-way ANOVA, P values indicated). UQ: Ubiquinone; MQ-4: menaquinone.