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. 2015 Dec 23;68(1):120–130. doi: 10.1080/01635581.2016.1115094

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© 2016 The Author(s). Published with license by Taylor & Francis Group, LLC

© 2016 Anupam Bishayee, Animesh Mandal, Piyali Bhattacharyya, and Deepak Bhatia

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 5. — Effects of pomegranate emulsion (PE) treatment on transcriptional expressions of genes related to apoptosis in 7,12-dimethylbenz(a)anthracene (DMBA)-initiated rat mammary tumorigenesis. Rats were sacrificed 18 wk following the commencement of the study and mammary tumor samples were harvested from various experimental groups. Total RNA was extracted. The resultant complementary DNA following reverse transcription was subjected to PCR using specific sequences for various genes: BAX, BCL2, BAD, CASPASE 3 (CASP3), CASPASE 7 (CASP7), CASPASE 9 (CASP 9), poly (ADP-ribose) polymerase (PARP), and cytochrome c (CYT. C). Representative reverse-transcriptase-PCR pictures are shown with glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as the housekeeping gene.