Skip to main content
. Author manuscript; available in PMC: 2016 Mar 9.
Published in final edited form as: Clin Immunol. 2015 Nov 6;162:37–44. doi: 10.1016/j.clim.2015.11.002

Fig. 2.

Fig. 2

No impact of Cp40 on the binding of human xenoreactive antibodies to iPECs. Binding of human xenoreactive antibodies (IgG/IgM) to iPECs was measured by FACS (A and B) and immunofluorescent staining (C, ×100) after the incubation of iPECs with either 20% HINHS (N) or Cp40-pretreated 20% HINHS (Cp40). iPECs incubated with secondary antibody alone served as negative controls (Control). For FACS analysis, the Gmean was used to evaluate the degree of xenoantibody binding to iPECs. (A) FACS results shown are representative of three independent experiments. (B) The extent of human IgG and IgM binding to iPECs is shown in the bar graphs. Data shown are means ± SEM (NS, P > 0.05 vs. N group; n = 3 per group). (C) Images are representative of three independent experiments.