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. Author manuscript; available in PMC: 2016 Mar 9.
Published in final edited form as: Clin Immunol. 2015 Nov 6;162:37–44. doi: 10.1016/j.clim.2015.11.002

Fig. 4.

Fig. 4

Cp40 inhibits human serum-mediated CDC in a dose-dependent manner. PI-positive dead cells were detected by FACS after the incubation of iPECs with 20% NHS pretreated with various doses of Cp40 (final concentrations from 1.25 to 1280 μg/ml). iPECs incubated with 20% HINHS were used as negative controls. The percentage of the total cells that were PI-positive was used to evaluate the degree of cell death. (A) Representative FACS results for negative controls and Cp40 pretreatment at 0, 1.25, 10, 20, and 1280 μg/ml. (B) The percentages of iPEC lysis mediated by 20% NHS that had been pretreated with various doses of Cp40 are shown as a line graph. Data shown are means ± SEM (n = 3).