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. 2016 Feb 25;1(2):e85633. doi: 10.1172/jci.insight.85633

Figure 2. Degradome and posttranslational modifications associated with the peptidome found in the synovial fluid of patients with juvenile idiopathic arthritis and controls.

Figure 2

(A) Number of peptide cleavage sites by each specified protease as analyzed by MEROPS and CutDB, found in the synovial fluid of patients with juvenile idiopathic arthritis (JIA) and controls (data were compiled from 3 separate proteomic analyses). (B) Posttranslational modifications (PTMs), mapped on the sequenced peptides, present in the synovial fluid of patients with JIA (n = 7) and controls (n = 2). Pie charts illustrate the percentage of posttranslational modified peptides found in the synovial fluid of patients with JIA and controls (data were compiled from 3 separate proteomic analyses). (C) Quantification of the increased carbonyl content in the proteome of the synovial fluid from JIA patients as compared with controls. Equal amounts of total protein from the synovial fluid of each JIA patient and control (10–20 μg) were derivatized with the reagent dinitrophenylhydrazine (DNPH), and the total carbonyl content (as nanomoles/mg protein) was determined spectroscopically. Average readings (mean ± SD) from 3 separate experiments are shown as bar graphs. Data were analyzed by 1-way ANOVA (P < 0.005) followed by Tukey test.