Figure 4. Transthyretin and anti-transthyretin antibodies in the synovial fluid and sera of juvenile idiopathic arthritis patients and controls.
(A) Quantification of transthyretin (TTR) autoantibodies, as detected by ELISA, present in the synovial fluid from patients with juvenile idiopathic arthritis (JIA) and controls (data were compiled from 3 separate ELISAs, with each patient’s samples run in quadruplicate). Data were analyzed by 1-way ANOVA and Tukey test. (B) Quantification of TTR autoantibodies, as detected by ELISA, present in the sera of patients with JIA and controls (data were compiled from 3 separate ELISAs, with each patient’s samples run in quadruplicate). Data were analyzed by 1-way ANOVA and Tukey test. (C and D) Amount of TTR present in the synovial fluid and sera from patients with JIA and controls (data were compiled from 3 separate ELISAs, with each patient’s samples run in quadruplicate) Data were analyzed by 1-way ANOVA and Tukey test. (E) Correlation between TTR protein and anti-TTR antibodies (Spearman r = 0.72, ****P < 0.001). The patient ID for each circle is reported in Supplemental Figure 4. Mean ± SD. *P < 0.05; **P < 0.005; ****P < 0.001.
