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. 2016 Feb 25;1(2):e85633. doi: 10.1172/jci.insight.85633

Figure 6. Production of TNF-α and IFN-γ by T cells isolated from synovial fluid of juvenile idiopathic arthritis patients in response to transthyretin peptides.

Figure 6

(A) Primary sequence of transthyretin (TTR) peptides. Residues in red are posttranslational modified (M is oxidized, and K and P are hydroxylated). (B) Fold increase of CD4+ T cells producing TNF-α found in the synovial fluid (SF) of juvenile idiopathic arthritis (JIA) patients. Data are reported as fold increase in the number of TNF-α–positive cells following stimulation with TTR peptides over unstimulated cells (1 out of 2 representative experiments is shown). (C) Fold increase of CD4+ T cells producing IFN-γ found in the SF of JIA patients (one out of two representative experiments is shown). Data are reported as fold increase in the number of IFN-γ–positive cells following stimulation with TTR peptides over unstimulated cells. (D) Examples of FACS analysis of TNF-α– and IFN-γ–producing T cells from different JIA patients before and after stimulation with TTR peptides. HLA-DR type of patient 12 is DRB1*03:07/07:01 and that of patient 50 is DRB1*01:01/11:01.