Table 2. Amplification sizes in different Bartonella spp. for each of the three PCR approaches in study.

		PCR approaches (bp)
Microorganism1	Illness2	16S rRNA	its	fla
Carrion’s disease involved Bartonella spp.
B. bacilliformis	Carrion’s disease	438	545	937
B. rochalimae	Carrion’s disease 3	438	696	974
B. ancashensis	Carrion’s disease 4	438	590	940
Main Bartonella spp. causing human illness
B. alsatica	Endocarditis	438	654	NA
B. clarridgeiae	Cat scratch disease	438	692	997
B. elizabethae	Endocarditis	438	777	NA
B. henselae	Cat scratch disease	438	719	NA
B. grahamii	Retinitis	438	715	NA
B. quintana	Trench fever, Cat scratch disease	438	619	NA
B. vinsonii subsp. berkhoffi	Endocarditis	438	727	NA
B. vinsonii subsp. arupensis	Bacteremia	438	742	NA
Other Bartonella spp.5
B. bovis		438	525	NA
B. capreoli		438	5646	NA
B. coopersplainsensis		438	701	NA
B. koehlerae		438	677	NA
B. pachyuromydis		438	545	NA
B. queenslandensis		438	715	NA
B. schoenbuchensis		438	527	1008
B. silvatica		438	690	NA
B. taylorii		438	689	NA

^1.- The 32 currently recognized Bartonella species (including the three B. vinsonii subsp.) plus B. tamiae were considered.

^2.- Only the more relevant pathologies have been referenced here.

^3.- Described as one case of Oroya fever-like in a tourist returning from Peru [4].

^4.- Described as a cause of Peruvian Wart in children living in an endemic area [3,5].

^5.- Most of them isolated from animals, and some sporadically reported from human infections. Indicated are only those microorganisms that have the fla gene or that the amplified its product differing 20 bp or less respecting any of Bartonella spp. involved in Carrion’s disease.

^6.- Uncertain amplification (two gaps close to primer 3’ terminal).

NA: non amplified or non-predicted amplification.

Highlighted in bold when experimental amplification of the three PCR approaches in study were performed.