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. 2016 Mar 9;11(3):e0151284. doi: 10.1371/journal.pone.0151284

Table 1. Primer sequences, amplicon sizes and annealing temperatures for qPCR assays.

Genes Oligo sequence (5' to 3') Amplicon size, bp Access. No.** Annealing temp,°C
CCL21 F: GCTATCCTGTTCTCGCCTCG 222 NM_001038076.2 60
R: ACTGGGCTATGGCCCTTTTG
CCR7 F: ACCCTCGCTAGCTACCTCAA 293 NM_001024930.3 64
R: CGGTCTCTTGTCTTGGGGAC
CD22 F: ACCTCAGTTTCCAGCCCAAG 188 XM_003587236.2 64
R: CCTCATGGTCACAGACTCGC
CXCL13 F: AACCCTCAAGCCAAATGGACA 154 NM_001015576.2 60
R: AACCCGGAGCAGGAATGTTG
LTB F: TGGGAAGAGGAGGTCAGTCC 215 XM_002697371.2 62
R: TAGCTTGCCATAAGTCGGGC
MS4A1 F: GCGGAGAAGAACTCCACACA 206 NM_001077854.2 64
R: GGGTTAGCTCGCTCACAGTT
IL2RA F: GCACGGTCAGGCTTCAGAT 288 NM_174358.2 64
R: TTCTTGACTTCTTCTGGCCTTG
CD19 F: CTCCCATACCTCCCTGGTCA 127 NM_001245998.1 64
R: GCCCATGACCCACATCTCTC
POU2AF1 GAGACCATGGTGACTGGTGG 246 NM_001075915.1 62
AATACGGCCATTGTGGGGAG
SH2D1A CAGCACCGGGGGTACATAAA 146 NM_001034733.2 62
TCCTGTAGCACCTTGTGTACTT
β-actin* F: CTAGGCACCAGGGCGTAATG 177 AF191490.1 60
R: CCACACGGAGCTCGTTGTAG

* Primer sequence from [69]

**NCBI accession number.