Table 1. Primer sequences, amplicon sizes and annealing temperatures for qPCR assays.
Genes | Oligo sequence (5' to 3') | Amplicon size, bp | Access. No.** | Annealing temp,°C |
---|---|---|---|---|
CCL21 | F: GCTATCCTGTTCTCGCCTCG | 222 | NM_001038076.2 | 60 |
R: ACTGGGCTATGGCCCTTTTG | ||||
CCR7 | F: ACCCTCGCTAGCTACCTCAA | 293 | NM_001024930.3 | 64 |
R: CGGTCTCTTGTCTTGGGGAC | ||||
CD22 | F: ACCTCAGTTTCCAGCCCAAG | 188 | XM_003587236.2 | 64 |
R: CCTCATGGTCACAGACTCGC | ||||
CXCL13 | F: AACCCTCAAGCCAAATGGACA | 154 | NM_001015576.2 | 60 |
R: AACCCGGAGCAGGAATGTTG | ||||
LTB | F: TGGGAAGAGGAGGTCAGTCC | 215 | XM_002697371.2 | 62 |
R: TAGCTTGCCATAAGTCGGGC | ||||
MS4A1 | F: GCGGAGAAGAACTCCACACA | 206 | NM_001077854.2 | 64 |
R: GGGTTAGCTCGCTCACAGTT | ||||
IL2RA | F: GCACGGTCAGGCTTCAGAT | 288 | NM_174358.2 | 64 |
R: TTCTTGACTTCTTCTGGCCTTG | ||||
CD19 | F: CTCCCATACCTCCCTGGTCA | 127 | NM_001245998.1 | 64 |
R: GCCCATGACCCACATCTCTC | ||||
POU2AF1 | GAGACCATGGTGACTGGTGG | 246 | NM_001075915.1 | 62 |
AATACGGCCATTGTGGGGAG | ||||
SH2D1A | CAGCACCGGGGGTACATAAA | 146 | NM_001034733.2 | 62 |
TCCTGTAGCACCTTGTGTACTT | ||||
β-actin* | F: CTAGGCACCAGGGCGTAATG | 177 | AF191490.1 | 60 |
R: CCACACGGAGCTCGTTGTAG |
* Primer sequence from [69]
**NCBI accession number.