Fig 7. The role of the outer cell His residues in pH sensing.

(a) Predicted three-dimensional structure of RdAQP1 showing two His residues (His85 and His275) positioned on the loops of the protein and facing the outside of the cell. (b) P_f values of GFP::WT (wild type), GFP::RdAQP1 and GFP::RdAQP1 mutant,. Different lowercase letters above the bars denote significant differences for each pH level between fungal types (P < 0.05).