Table 2. Effect of HgCl2 on swelling of Rhizopus delemar spores incubated in sweet potato active fraction (SPAF) and germ-tube emergence.
Spores were treated with 40 μM HgCl2 to inhibit aquaporin activity. Additional treatment with the reducing agent 2-β-mercaptoethanol (2ME, 5 μM) was used to reverse the HgCl2 inhibition effect. The assays were performed at pH 4.7. Values are means ± SE (n = 500)
| Treatment | pH | Diameter (μm) | Germ-tube emergence (%) |
|---|---|---|---|
| SPAF | 4.7 | 23.1 ± 1.6 *A | 97.2 ± 1.2 A |
| SPAF + HgCl2 | 4.7 | 7.8 ± 0.6 B | 0 B |
| SPAF + HgCl2 + 2ME | 4.7 | 20.5 ± 0.4 A | 98.2 ± 1.4 A |
* Within each measured parameter different upper case letters are significantly different from each other (P≤ 0.05).