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. 2016 Mar 9;11(3):e0150745. doi: 10.1371/journal.pone.0150745

Fig 7. The effects of gemigliptin on NADPH oxidase and Akt and FoxO3a pathways in the kidneys of db/db mice.

Fig 7

Representative Western blot analyses of NADPH oxidase (NOX4, p67-phox, and p47-phox) levels and the estimates of ROS-induced injury (including iNO levels) are shown (A and B). NADPH oxidases and ROS-associated enzymes were quantitated using NIH ImageJ software (C and D). Quantitative assessments of urinary 24-h 8-OH-dG levels in each group are shown (E). Representative Western blot showing PI3K, total Akt, phosphor-Akt, total FoxO3a, phosphor-FoxO3a, and β-actin bands (F). The densitometric graphs displaying PI3K/β-actin, pAkt/tAkt, and pFoxO3a/tFoxO3a protein expression ratios derived using NIH ImageJ software (G-I). β-actin served as the internal control. All results are expressed as means ± SEMs. *p < 0.05, **p < 0.01 vs. db/m; #p < 0.05, ###p < 0.001 vs. db/db.