Table 2. Quantitative HPLC analyses of phenolic compounds in the Cudrania tricuspidata stem (CTS) extract.
Phenolic | Peak area | Contents of phenolic compound (mg/g) | ||||||
---|---|---|---|---|---|---|---|---|
compounds | #1 | #2 | #3 | #1 | #2 | #3 | Average | SD |
Chlorogenic acid | 89,062.62 | 93,515.91 | 92,651.37 | 62.54 | 65.67 | 65.06 | 64.42 | 1.66 |
(+)-Catechin | 484.22 | 431.78 | 424.37 | 1.04 | 0.94 | 0.93 | 0.97 | 0.06 |
Caffeic acid | 37,200.83 | 37,207.60 | 36,255.37 | 12.21 | 12.21 | 11.90 | 12.11 | 0.18 |
Phloretic acid | 1,378.75 | 1,559.26 | 1,425.37 | 2.72 | 3.08 | 2.82 | 2.88 | 0.19 |
Veratric acid | 22,186.75 | 22,509.57 | 22,365.37 | 14.04 | 14.24 | 14.15 | 14.14 | 0.10 |
Hesperidin | 10,541.11 | 10,737.77 | 10,657.37 | 7.45 | 7.59 | 7.54 | 7.53 | 0.07 |
Quercetin | 1,751.90 | 1,832.04 | 1,789.37 | 1.43 | 1.49 | 1.46 | 1.46 | 0.03 |
Naringenin | 2,255.98 | 2,493.72 | 2,565.37 | 0.77 | 0.86 | 0.88 | 0.84 | 0.06 |
Total | 102.21 | 106.10 | 104.74 | 104.35 | 1.97 |
The seventeen kinds of the phenolic acid composition of the sample were analyzed by comparing the spectrum of the sample and standards components matched to create a standard curve from Peak area per component to quantify the amount of change. Quantitative HPLC analyses of phenolic compounds were performed three times.