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. 2016 Mar 9;11(3):e0149331. doi: 10.1371/journal.pone.0149331

Fig 3. Spectroscopic Analysis (FTIR) identifies miR-15a/16-1 differences.

Fig 3

A) FTIR absorption spectra of spleen cells (NZB, repeated three times). Cells were air-dried on an area of 4mm2 on CaF2 coated surface. B) 2nd order derivative spectra of spleen cells in 1200-850cm-1 range. Spectral variations were observed between the non-NZB (DBA strain no CLL) with high miR-15a/16-1 levels when compared to the NZB spleen source. These were observed at 883.82 and 1028.4 cm-1 indicated by arrows. C) Hierarchical clustering was performed to investigate the effect of microRNA miR-15a/16-1 levels in spleen cells from DBA (high miR-15a/16-1) versus NZB (low miR-15a/16-1) using Ward’s algorithm and 2nd order derivative spectra at 883.82 cm-1 and 1028cm-1.