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. 2016 Mar 9;11(3):e0150701. doi: 10.1371/journal.pone.0150701

Fig 6. HapX of A. benhamiae under high iron concentrations.

Fig 6

(A) Cultivation of A. benhamiae wild type, ΔhapX mutant and hapXC reconstituted strain during iron starvation (-Fe), high iron concentrations (1–10 mM FeSO4) and in the presence of the iron chelator BPS on solid medium for 7 d at 30°C. Scale bar represents 5 mm. (B) Cultivation of A. benhamiae wild type, ΔhapX mutant and hapXC reconstituted strain in AMM with high iron concentrations (1–7 mM FeSO4). Data represent the means and standard deviations of three biological replicates. The differences between wild type and ΔhapX mutant were statistically significant between 5 mM and 7 mM Fe (2way ANOVA; ** significant at P < 0.01, *** significant at P < 0.001). (C) Quantitative RT-PCR analysis of the cccA gene under different iron concentrations. The mycelium was cultivated under high iron concentrations (hFe) or shifted for 1 h from -Fe to +Fe (sFe). Data represent the means and standard deviations of three biological replicates. The differences between wild type and ΔhapX mutant were statistically significant during sFe (2way ANOVA; *** significant at P < 0.001).