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Inhibition of choline uptake in S. cerevisiae by G25. Choline transport in the wild-type strain of S. cerevisiae was measured as described in Materials and Methods. The uptake of 1 μM [methyl-3H]choline in the presence of 100 μM ethanolamine (Etn) and a 4-, 20-, and 100-fold excess of unlabeled choline, G25, and T16 is shown as a percentage of the counts obtained in the control (Ctrl) without drugs, choline, or ethanolamine.
