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Ni-chelate affinity chromatography. (A) For kinase assays, 5 μl of each fraction was assayed for histone H1 kinase activity. The reaction mixtures after the reaction was stopped were subjected to SDS-PAGE and autoradiography. (B) For Western blotting, 20 μl of each fraction (or total lysate) was separated by SDS-PAGE and subjected to immunoblotting, using an antiserum specific for CRK3. Lane numbering refers to the fraction number, and L indicates the total lysate.
