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Sanger sequencing of HRAS showed the previously identified c.64 C>A (p.Gln22Lys), present in heterozygous state in the splenic tissue derived DNA, but skewed in the pancreatic nodule. Sequencing in both directions shows an increase of the mutant allele over the wild-type. [Color figure can be seen in the online version of this article, available at http://wileyonlinelibrary.com/journal/ajmga].
