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. Author manuscript; available in PMC: 2016 Mar 9.
Published in final edited form as: Sci Transl Med. 2015 May 6;7(286):286ra67. doi: 10.1126/scitranslmed.aaa1652

Fig. 2. Biliatresone tissue specificity.

Fig. 2

(A and B) Histological cross sections showing severe morphological defects of the gallbladder (asterisks) and cystic duct (arrows) in an 8 dpf biliatresone–treated larva (B) compared with a control larva (A). (C and D) Normal histological appearance of hepatocytes and liver sinusoids in an 8 dpf toxin-treated larva (D) compared with a control larva (C). Sinusoids (containing nucleated red blood cells; arrows) are the principal vascular channel in the liver of zebrafish larvae; thus, branches of the portal vein and artery are not seen in these histological sections. Intrahepatic bile ducts are too small to be seen. (E to H) Bright-field images of live 8 dpf control (E) and toxin-treated (F) Tg(lfabp-RFP) larvae with corresponding fluorescent images of the liver (G and H). Arrows, liver (E and F). Scale bars, 10 µm (A to D); 200 µm (E to H).