Table 1.
Colchicine concentration (%) | Duration (exposure) time (h) | Treatment number | Survival number | Variation number | Variation rates (%) | |
---|---|---|---|---|---|---|
| ||||||
Of initial buds | Of survival buds | |||||
0.05 | 12 | 40 | 84 | 14 | 34.89 ± 1.35 | 16.36 ± 1.29 |
24 | 40 | 71 | 21 | 52.45 ± 2.43 | 29.43 ± 0.92 | |
36 | 40 | 68 | 24 | 60.32 ± 2.89 | 33.94 ± 3.01 | |
48 | 40 | 65 | 24 | 59.56 ± 2.51 | 36.92 ± 1.02 | |
60 | 40 | 70 | 30 | 75.64 ± 2.78 | 41.27 ± 1.37 | |
72 | 40 | 61 | 34 | 85.65 ± 1.1 | 54.73 ± 2.13 | |
84 | 40 | 54 | 21 | 52.53 ± 2.01 | 38.89 ± 1.32 | |
0.1 | 12 | 40 | 57 | 19 | 47.59 ± 3.92 | 33.33 ± 3.19 |
24 | 40 | 42 | 16 | 40.32 ± 4.02 | 38.09 ± 3.89 | |
36 | 40 | 37 | 19 | 47.57 ± 4.62 | 51.35 ± 4.58 | |
48 | 40 | 45 | 22 | 55.68 ± 3.85 | 48.88 ± 3.58 | |
60 | 40 | 49 | 24 | 60.56 ± 5.01 | 48.97 ± 4.97 | |
72 | 40 | 35 | 20 | 50.56 ± 4.53 | 57.14 ± 4.62 | |
84 | 40 | 29 | 15 | 37.52 ± 3.59 | 51.72 ± 3.57 | |
0.2 | 12 | 40 | 45 | 20 | 50.35 ± 4.69 | 43.44 ± 5.09 |
24 | 40 | 35 | 17 | 42.54 ± 4.82 | 46.57 ± 5.01 | |
36 | 40 | 35 | 21 | 52.55 ± 3.78 | 60.75 ± 3.27 | |
48 | 40 | 31 | 20 | 50.29 ± 5.96 | 65.51 ± 4.91 | |
60 | 40 | 29 | 13 | 32.54 ± 4.82 | 42.82 ± 5.14 | |
72 | 40 | 23 | 12 | 30.19 ± 4.27 | 52.31 ± 4.78 | |
84 | 40 | 21 | 11 | 27.45 ± 4.87 | 52.38 ± 3.69 |
Induction on MS solid medium, and the seedlings transplanted in soil for 6 months.
The seedlings both from the lateral buds and the cluster buds were in the statistical range.