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. 2016 Mar 1;66(2):169–174. doi: 10.1270/jsbbs.66.169

Table 1.

Effect of different concentrations and treatment duration of colchicine on polyploidy induction (mean ± standard error) in P. cablin (all data are in percentages)

Colchicine concentration (%) Duration (exposure) time (h) Treatment number Survival number Variation number Variation rates (%)

Of initial buds Of survival buds
0.05 12 40 84 14 34.89 ± 1.35 16.36 ± 1.29
24 40 71 21 52.45 ± 2.43 29.43 ± 0.92
36 40 68 24 60.32 ± 2.89 33.94 ± 3.01
48 40 65 24 59.56 ± 2.51 36.92 ± 1.02
60 40 70 30 75.64 ± 2.78 41.27 ± 1.37
72 40 61 34 85.65 ± 1.1 54.73 ± 2.13
84 40 54 21 52.53 ± 2.01 38.89 ± 1.32
0.1 12 40 57 19 47.59 ± 3.92 33.33 ± 3.19
24 40 42 16 40.32 ± 4.02 38.09 ± 3.89
36 40 37 19 47.57 ± 4.62 51.35 ± 4.58
48 40 45 22 55.68 ± 3.85 48.88 ± 3.58
60 40 49 24 60.56 ± 5.01 48.97 ± 4.97
72 40 35 20 50.56 ± 4.53 57.14 ± 4.62
84 40 29 15 37.52 ± 3.59 51.72 ± 3.57
0.2 12 40 45 20 50.35 ± 4.69 43.44 ± 5.09
24 40 35 17 42.54 ± 4.82 46.57 ± 5.01
36 40 35 21 52.55 ± 3.78 60.75 ± 3.27
48 40 31 20 50.29 ± 5.96 65.51 ± 4.91
60 40 29 13 32.54 ± 4.82 42.82 ± 5.14
72 40 23 12 30.19 ± 4.27 52.31 ± 4.78
84 40 21 11 27.45 ± 4.87 52.38 ± 3.69
a

Induction on MS solid medium, and the seedlings transplanted in soil for 6 months.

b

The seedlings both from the lateral buds and the cluster buds were in the statistical range.