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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Bioorg Med Chem Lett. 2016 Feb 18;26(7):1799–1802. doi: 10.1016/j.bmcl.2016.02.038

Figure 2.

Figure 2

7-EAA is taken up by HeLa cells and incorporated into cellular RNA. A) Detection of biotin-labeled RNA after click reaction with biotin-azide (M) biotinylated molecular weight markers in kb, (1) Sample isolated from cells grown in absence of 7-EAA, treated with RNase V1; (2) Sample isolated from cells grown in the presence of 100 μM 7EAA, treated with RNase V1; (3) Sample isolated from cells grown in the absence of 7-EAA, no RNase V1 treatment; (4) Sample isolated from cells grown in presence of 100 μM 7-EAA, no RNase V1 treatment. B) Same as in A) with ethidium bromide (EtBr) detection instead of biotin detection.