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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Bioorg Med Chem Lett. 2016 Feb 18;26(7):1799–1802. doi: 10.1016/j.bmcl.2016.02.038

Figure 3.

Figure 3

Synthesis of 17 nt RNA by in vitro transcription with 7-EAATP and T7 RNA polymerase. A) T7 RNA polymerase transcription template for synthesis of 17 nt RNA containing one 7-EAA or A residue (X). B) Products of T7 polymerase transcriptions with template shown in (A) containing 5 mM ATP or 7-EAATP, α -32P GTP and allowed to proceed for 2 h at 37 °C. (arrow indicates major transcription product). C) CuAAC reaction with 17 nt RNAs. Lanes 1-3: product from transcription with 7-EAATP, Lanes 4-6: product from transcription with ATP. Lanes 1 and 4: no CuAAC reaction reagents added, Lanes 2 and 5: CuAAC reagents lacking azide, Lanes 3 and 6: CuAAC reagents plus azide. (*) indicates position of CuAAC product.