TABLE 1.
Mean LPV IC50,total, % free LPV, and derived LPV IC50,free in medium containing 5 to 50% FCS
| % FCS in culture medium | LPV IC50,total (ng/ml) | LPV free fraction (%)a | LPV IC50,free (ng/ml)b |
|---|---|---|---|
| 5 | 11 ± 7 | 6.3 ± 0.7 | 0.69 ± 0.44 |
| 10 | 17 ± 4 | 3.9 ± 0.4 | 0.68 ± 0.14 |
| 20 | 43 ± 15 | 1.5 ± 0.1 | 0.64 ± 0.23 |
| 50 | 119 ± 21 | 0.6 ± 0.1 | 0.77 ± 0.14 |
a
Free fraction of 0.1-μg/ml LPV for each FCS concentration (mean ± SD; n = 3).
b
Calculated as the product of the mean LPV free fraction and the individual LPV IC50,total (mean ± SD; n = 2). The mean LPV IC50,free for all FCS concentrations was 0.69 ± 0.06 ng/ml.