TABLE 2.
Mean RTV IC50,total, % free RTV, and derived RTV IC50,free in medium containing 5 to 50% FCS
| % FCS in culture medium | RTV IC50,total (ng/ml) | RTV free fraction (%)a | RTV IC50,free (ng/ml)b |
|---|---|---|---|
| 5 | 53 ± 26 | 5.7 ± 1.1 | 3.0 ± 1.5 |
| 10 | 89 ± 14 | 5.6 ± 1.8 | 5.0 ± 0.8 |
| 20 | 141 ± 40 | 2.7 ± 0.6 | 3.8 ± 1.1 |
| 50 | 318 ± 66 | 1.3 ± 0.1 | 4.0 ± 0.8 |
a
Free fraction of 0.1-μg/ml RTV for each FCS concentration (mean ± SD; n = 3).
b
Calculated as the product of the mean RTV free fraction and the individual RTV IC50,total (mean ± SD; n = 3). The mean RTV IC50,free for all FCS concentrations was 4.4 ± 0.8 ng/ml.