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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Mucosal Immunol. 2015 Sep 9;9(3):718–729. doi: 10.1038/mi.2015.95

Figure 2. Blockade of IL-17A in A/J mice attenuates the host immune response during pulmonary L. pneumophila infection.

Figure 2

A-B. A/J mice were treated intratracheally with IL-17A blocking antibody (1 μg/mouse) or control IgG 1 h before L. pneumophila (107 CFU/mouse) infection. Unlavaged lung (A) and liver (B) were isolated and homogenized and bacterial CFUs enumerated. C-D. BALF was obtained at 48 and 72 h post-L. pneumophila inoculation from blocking Ab or control Ab-treated mice, and cell enumeration was performed to determine total WBC (C) and neutrophil infiltration (D). E-F. Chemokine levels in BALF were measured by sandwich ELISA following infection with L. pneumophila and Ab blockade. (n=6-8 mice/group). *, p<0.05; **, p<0.01;***, p<0.001; (significance as compared to L. pneumophila-infected C57Bl/6 mice).