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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Mucosal Immunol. 2015 Sep 9;9(3):718–729. doi: 10.1038/mi.2015.95

Figure 3. Blockade of IL-17F in A/J mice attenuates the host immune response during pulmonary L. pneumophila infection.

Figure 3

A-F. A/J mice were treated intratracheally with IL-17F blocking antibody (1 or 10 μg/mouse) or control IgG 1 h after L. pneumophila (107 CFU/mouse) inoculation. Unlavaged lung (A) and liver (B) were isolated and tissues were homogenized. The lung or liver homogenates were used to enumerate the bacterial CFU. C2-D2. BALF was obtained post-L. pneumophila inoculation from Ab treated mice, and cell enumeration was performed to determine total WBC (C) and neutrophil recruitment (D). E-F. Chemokine levels in BALF were measured by sandwich ELISA following antibody blockade and infection with L. pneumophila. For experiments A2-F2, (n=6-8 mice/group). *, p<0.05; **, p<0.01;***, p<0.001; (significance as compared to L. pneumophila-infected C57Bl/6 mice). Cont-Ab: Isotype-matched control antibody.