Skip to main content
. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Mucosal Immunol. 2015 Sep 9;9(3):718–729. doi: 10.1038/mi.2015.95

Figure 9. Human neutrophils produce IL-17A following L. pneumophila infection.

Figure 9

Bacterial (Gram-negative) pneumonic or non-pneumonic lung sections were stained with lipocalin-2 (lipo; neutrophil-specific) Ab, IL-17A Ab, or control Ab and 500 cells in each section were counted. Shown is a representative section of five lungs from 5 pneumonic or normal in each group (A) and the mean ± SE of 500 cells from 5 slides (B). Blood was obtained from healthy volunteers and neutrophils were purified using negative selection with magnetic beads. Neutrophils were stimulated with L. pneumophila at a MOI of 1.0. At 3 h postinfection, neutrophils were stained with Abs against RORγt and IL-17A and immunofluorescence performed. Shown is a representative image of neutrophils from 5 healthy individuals (C) and the mean ± SE of 500 cells from 5 slides (D). Production of IL-17A and IL-17F by neutrophils following L. pneumophila infection. For A-D, p<0.05; **, p<0.01;***, p<0.001. E. Human neutrophils were purified and stimulated with L. pneumophila at an MOI of 1.0. At 3 h postinfection, neutrophils were stained with IL-17A antibody or IL-17F antibody. The upper panel indicates control or uninfected cells whereas the lower panel indicates stimulated cells. Dot plot shows IL-17A and/or IL-17F producing cells upon stimulation.