Fig. 5.

Relative expression levels of PABPC1 and PABPC3 proteins in the SC fraction. We used Western blot technique to determine relative expression profiles of these proteins. a Western blot results of the PABPC1 and PABPC3 proteins in the SC and RS fractions were given. Both PABPC1 and PABPC3 proteins had higher expression levels in the SC fraction obtained from hyposperm group than that of the SC fraction isolated from either RS arrest or SC arrest group. b ImageJ analysis was performed to further evaluate the Western blot results of the PABPC1 protein. We found that the SC fraction isolated from hyposperm group possessed significantly higher PABPC1 protein expression when compared to the SC fractions from RS arrest and SC arrest groups (P < 0.05). c Relative expression of PABPC3 protein in the SC fraction was also analyzed by using ImageJ software. We revealed that the SC fraction from hyposperm group expressed the PABPC3 at higher levels in the hyposperm group in comparison to the SC fractions from remaining NOA groups (P < 0.05). The statistical significance among groups was analyzed by using one-way ANOVA Dunnett’s T3 post hoc test, and their P values were presented on columns using line charts. Note that P < 0.05 was considered as statistically significant. Data are presented as mean ± SEM