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. Author manuscript; available in PMC: 2016 Mar 10.
Published in final edited form as: Dis Aquat Organ. 2015 Feb 10;113(1):33–40. doi: 10.3354/dao02830

Fig. 3.

Fig. 3

PCR detection of pirA- and pirB-like genes. (A) Amplifications with pathogenic acute hepatopancreatic necrosis disease (AHPND)-Vibrio parahaemolyticus strains and non-pathogenic strains. Pathogenic strains collected from Mexico (Lane M: 1 kb plus DNA ladder; Lane 1: strain 13-511/A1; Lane 3: strain 13-306/D4, see Table 2) and Vietnam (Lane 2: strain 13-028/A3; Lane 4: strain 12-194G); non-pathogenic strains from shrimp farms in Vietnam (Lane 5: strain 13-028/A2), India (Lane 6: strain 13-488L), and USA (Lane 7: strain 13-431). (B) Determination of sensitivity by serially diluting V. parahaemolyticus 13-028/A3 DNA; bacterial concentrations were as follows: 1 × 107 (Lane 1), 1 × 106 (Lane 2), 1 × 105 (Lane 3), 1 × 104 (Lane 4), and 1 × 103 CFU ml−1 (Lane 5), and a non-template control (Lane 6). Lane M: 1 kb plus DNA ladder