TABLE 1.
Bacterial strains and plasmids used in this study
| S. aureus strains | Characteristicsa | Source or reference |
|---|---|---|
| RN450 (NCTC 8325-4) | Laboratory strain of S. aureus cured of all the prophages | 29 |
| RN450PmsrA1::lacZ | S. aureus RN450; a reporter strain for the msrA1 promoter (Ermr) | 43 |
| RN4220 | Restriction minus derivative of S. aureus RN450 | 22 |
| COL | Methicillin-resistant (ho) S. aureus | 35 |
| COL::msrA1 | S. aureus COL with mutation in the msrA1 gene (Kanr) | This study |
| SH1000 | Functional rsbU derivative of S. aureus RN450 | 20 |
| SH1000PmsrA1::lacZ | Reporter strain for the msrA1 promoter (Ermr) | This study |
| BB270 | Methicillin-resistant (he) S. aureus | 14 |
| BB270PmsrA1::lacZ | Methicillin-resistant msrA1 promoter reporter strain (Ermr) | This study |
| 13136p−m+ | Methicillin-resistant (he) S. aureus | 35 |
| DU4916 | Methicillin-resistant (ho) S. aureus | 14 |
| RN6911agr | agr mutant of S. aureus RN6390 (Tetr) | 41 |
| RN6911PmsrA1::lacZ | agr msrA1 mutant promoter reporter strain (Tetr Ermr) | This study |
| ALC136 | sar mutant of S. aureus RN6390 (Ermr) | 41 |
| H | Laboratory strain of S. aureus | |
| Wood 46 | Laboratory strain of S. aureus | |
| RN7497 | Laboratory strain of S. aureus | This study |
a
Kanr, kanamycin resistant; Ermr, erythromycin resistant; Tetr, tetracycline resistant; he and ho, hetero- and homogeneous, respectively.