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. 2016 Mar 4;7:10778. doi: 10.1038/ncomms10778

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(a,d) Strategy for labelling ER membrane (Halo-Sec61β) and ER lumen (SNAP-KDEL). COS-7 cells expressing the above mentioned fusion proteins were then labelled with 590-BG and SiR-CA (a–c) and 590-CA and SiR-BG (d–f). (c,f) Magnified regions of interest (indicated by white boxes) from b and e, respectively. Scale bars, b,e=2 μm; c,f=200 nm. Imaging was performed at 22 °C. All images were deconvolved and corrected for bleaching.