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. 2016 Feb 25;2016:5326382. doi: 10.1155/2016/5326382

Figure 2.

Figure 2

PNS increased cell viability in rat RCECs exposed to high glucose. Seeded in 24-well or 96-well plates, the rat RCECs were incubated with varying concentrations of PNS (20, 50, 100, and 200 μg/mL) in 30 mM glucose. After culturing for 24 h, 48 h, or 72 h, MTT and trypan blue staining assay were used to examine cell viability. The MTT assay showed that 50, 100, and 200 μg/mL of PNS increased cell viability after 48 and 72 h (a). The trypan blue assay showed that 100 and 200 μg/mL of PNS increased cell viability after 48 h and that 100 μg/mL of PNS increased cell viability after 72 h (b). The experiment was repeated three times. Data are expressed as the mean ± SD (n = 5). ## P < 0.01 versus 5.5 mM glucose; ∗∗ P < 0.01 and ∗ P < 0.05 versus 30 mM glucose.