TABLE 2.
Primers used to amplify junction region between each plasmid replicon and the blaCMY-2 region
| Region | Primer sequencea | Amplicon size (bp) |
|---|---|---|
| pNF1358 upstream junction (type B) | For, GTCATCAGACCTGTGCG | 505 |
| Rev, CGGAAATATCAAACTCGT | ||
| pNF1358 downstream junction (type B) | For, GTGTATTTCAGGCCAATCGC | 659 |
| Rev, CTGGGTATTCTCTGGTGCA | ||
| pNF4656 upstream junction (type C) | For, GACACCTTGCCGTTAATC | 523 |
| Rev, CGGAAATATCAAACTCGT | ||
| pNF4656 downstream junction (type C) | For, GCGAGACGCACTCCAGTC | 1,378 |
| Rev, CTGTTGCAAATAGTCGGTG | ||
| pIW759 upstream junction (type D) | For, CGCTGAACATGAAAGGA | 706 |
| Rev, CGGAAATATCAAACTCGT | ||
| pIW759 downstream junction (type D) | For, GCGAGACGCACTCCAGTC | 641 |
| Rev, GCTGTATACGCAGTGCTTT |
a
Abbreviations: For; forward primer. Rev; reverse primer.