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. 2016 Mar 10;6:22807. doi: 10.1038/srep22807

Figure 2. The amino-terminal domain (ATD) of hTAS1R2 is required for sensitivity to MCL.

Figure 2

HEK293 cells were transiently transfected with full length and/or chimera of TAS1R2 and TAS1R3, and Gα16-gust44. TAS1R2 (left) and TAS1R3 (right) are shown by schematic receptors. ATD, amino-terminal domain; CRD, cysteine-rich domain; TMD, transmembrane domain. The full length and the chimera of TAS1Rs are indicated by three letters including of H (human; red) or M (mouse; blue) (example, chimera containing the ATD and the CRD of human receptor coupled to the TMD of mouse receptor as for HHM). The responses of the receptors to 3 mM citric acid (CA: pH 5.0) before and after application of MCL (10 μg/ml) were examined. 15–30 cells. Data are expressed as means ± S.E.