Table 3. Intra-day (n = 6, at each QC level) and inter-day (n = 18, Six QCs at each level/day for 3 different days) assay precision and accuracy for fipronil, fipronil sulfone and fipronil desulfinyl in rat and human blood.

Species	Compound	Concentration(ng/mL)	aPrecision (RSD)		bAccuracy (%)
			Intra-day	Inter-day	Intra-day	Inter-day
Rat	Fipronil	0.1	6.02	7.46	94.53	95.06
		0.3	7.93	8.89	94.17	97.93
		40	2.07	10.02	105.79	101.86
		80	2.77	5.95	108.65	107.13
	Fipronil Sulfone	0.1	6.93	7.36	102.88	99.30
		0.3	8.99	7.62	105.39	105.70
		40	3.58	8.25	97.92	96.51
		80	4.34	8.06	93.48	103.73
	Fipronil Desulfinyl	0.1	9.95	9.88	101.03	102.82
		0.3	6.32	9.57	103.78	96.65
		40	3.65	7.33	93.21	99.38
		80	5.67	10.05	98.79	104.34
Human	Fipronil	0.1	6.07	8.78	100.13	98.16
		0.3	5.29	7.37	92.61	95.24
		40	2.60	4.50	97.88	99.82
		80	6.98	5.09	105.27	105.08
	Fipronil Sulfone	0.1	7.31	7.96	100.35	98.22
		0.3	5.28	6.78	103.17	99.61
		40	1.93	3.02	98.38	98.51
		80	2.68	3.23	107.67	107.31
	Fipronil Desulfinyl	0.1	5.34	8.14	101.02	99.57
		0.3	7.45	7.45	105.28	101.67
		40	2.45	4.00	96.42	98.01
		80	4.89	4.84	107.29	107.19

^aExpressed as % R.S.D. (S.D./mean) × 100.

^bCalculated as (mean determined concentration/nominal concentration) × 100.