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. 2016 Mar 10;6:22861. doi: 10.1038/srep22861

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Copyright © 2016, Macmillan Publishers Limited

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(a) Full-length HA-Odf2 was transfected into 293T cells either alone or in combination with Myc-Tssk4, and the electrophoretic migration rates changed for both Odf2 and Tssk4 (2^nd, 3^rd, and 4^th lanes) when co-expressed compared with the singly transfected Odf2 (1^st lane) and Tssk4 (5^th lane). (b,c) Full-length HA-Odf2 was transfected into 293T cells either alone or together with two kinase-dead mutants, including (b) Myc-Tssk4 (K54M) and (c) Myc-Tssk4 (T197A). The electrophoretic migration rates of the Odf2 and Tssk4 mutants did not change. All the experiments including cell transfection, SDS-PAGE and Western blot were performed under the same experimental conditions. Since there is great molecular weight gap between HA-Odf2 (about 70kD) and Myc-Tssk4 (about 35kD), the blots are cropped to improve the clarity and conciseness of the presentation. The Western blot in all the other figures were showed in the same way.