Figure 4. Phosphorylation of Odf2 at Ser-76 in vivo.
(a) Phospho-Ser-76 of Odf2 was detected in mouse sperm lysate using a specific antibody. Three different adult C57BL-6 mouse were used. (b) Analysis of the phosphorylation sites via LC-MS/MS. The MS/MS spectrum of the phosphorylated peptide 64-NPPHCLEITPPSSEK-80 displays a series of b- and y-ions, indicating that the underlined serine residue is a putative phosphorylation site.