Table 2. SAXS data collection and derived parameters.

Data collection parameters
Beamline	EMBL beamline X33 (DORIS III, DESY)48
Wavelength (Å)	1.5
s range (Å−1)	0.01–0.6
Exposure time (s)	120
Concentration range (mg/ml)	1.3–9.9
Temperature (°C)	12
Structural parameters
Rg (Å) (from Guinier)	30 ± 2
Rg (Å) (from p(r))	29 ± 3
Dmax (Å)	90 ± 10
Porod volume estimate (Å3)	145, 000 ± 15, 000
Molecular mass from Porod volume (kDa)	91 ± 9
Molecular mass from dummy atom modelling (kDa)	77 ± 8
Monomeric molecular mass calculated from sequence (kDa)	29.8
Software employed
Primary data reduction	SASFLOW pipeline49
Ab initio analysis	DAMMIF45
Rigid body modelling	CORAL23