FIG. 3.

Accumulation of oritavancin in J774 macrophages incubated for 2 h at 37°C with increasing extracellular concentrations of oritavancin in serum-free medium or in medium supplemented with 10 or 20% FCS. Results are given as arithmetic means ± standard deviations (n = 3) of the cellular concentrations recorded (micrograms of oritavancin per milligram of cell protein; the apparent accumulation ratio for each value of the extracellular concentration can be calculated by multiplying the values on the ordinate by 200 [based on a cell volume of 5 μl/mg of protein]). prot, protein.