Figure 7. Addition of exogenous cysteine and GSH restores cytochrome bd assembly in cydD cells.

Wild-type and cydD strains of E. coli BW25113 were harvested at 40 Klett and CO-difference spectra were recorded and normalized to give a common Soret peak magnitude. Exogenous cysteine (0.5 mM) and GSH (1 mM) were added to the growth media where indicated.