Table 3.
Effects of rutin on intestinal cytokines induced by methotrexate in rats
| Groups | Treatment | IL-2 (pg/ml) | IL-4 (pg/ml) | IL-10 (pg/ml) |
|---|---|---|---|---|
| Group-I | Sham control (Normal saline, 3.0 ml/kg) | 398.10 ± 5.32 | 162.18 ± 8.79 | 63.09 ± 5.51 |
| Group-II | Toxic control (MTX, 2.5 mg/kg) | 671.93 ± 56.65a | 213.79 ± 7.64a | 88.57 ± 3.45a |
| Group-III | Rutin (50 mg/kg) | 174.15 ± 8.20***a | 193.50 ± 1.47***a | 104.05 ± 0.65**a |
| Group-IV | Rutin + MTX(50 + 2.5 mg/kg) | 233.52 ± 14.69***a | 117.29 ± 0.023***a | 63.21 ± 2.96 |
| Group-V | Rutin (100 mg/kg) | 178.72 ± 3.28***a | 198.26 ± 7.76***a | 122.11 ± 1.88***a |
| Group-VI | Rutin + MTX (100 + 2.5 mg/kg) | 272.02 ± 15.00***a | 179.24 ± 1.57*** | 96.99 ± 1.50***a |
Each group contains six animals. Values are represented as mean ± SEM
Statistical significance compared to toxic control using one-way ANOVA followed by Bonferroni test
*P < 0.05, **P < 0.01, and ***P < 0.001 were considered statistically significant
Statistical significance compared to normal control (Group-I) using one-way ANOVA followed by Bonferroni test
c P < 0.05, b P < 0.01 and a P < 0.001 were considered statistically significant