Fig. 11.

SorCS2 expression in cardiovascular system, skin and adrenal gland. Immunohistofluorescence was performed on sagittal sections (10 μm) of embryos on embryonic day E13.5, E15.5 and E17.5. SorCS2 is weakly expressed in the developing ventricle in a subset of cells (a, b). Medium-large arteries show prominent SorCS2 IR while veins show weak IR (c-e). In the skin SorCS2 is found localized to the deep dermal layers (f, i). Lastly, the adrenal gland shows pronounced expression of SorCS2 in a large portion of the cells (g) but by E17.5 this localization has changed to the periphery of the gland, analogous to the kidney (h). Green channel is SorCS2, red channel is TH (g) or calbindin (h) and blue channel is Hoechst nuclear stain. Scale bars = 50 μm. Ad, adrenal gland; Ar, arteries; De, dermis; Ep, epidermis; Ki, kidney; Lu, lung; Ve, vein; Vt, ventricle of heart