Fig. 2.

Sortilin (−/−) embryos might have fewer and larger lung branches. Preliminary data. Embryos were taken at E15.5 from WT and Sortilin embryos, and cut at 10 μm thick slices on a cryotome and stained for TTF-1 (Red channel) and Hoechst (Blue channel). a shows images from WT lung at E15.5 and b shows lung from a Sortilin(−/−) embryo. The bronchial branches appear larger and fewer in the knockout compared to the WT, resembling a more immature lung (see pictures from E13.5 in Fig. 1 for comparison). Scale bars = 100 μm