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. 2016 Mar 10;17:8. doi: 10.1186/s12860-016-0085-9

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© Boggild et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Sortilin is expressed in costae, vertebrae and brown adipose tissue. Immunohistofluorescence was performed on sagittal sections (10 μm) of embryos on embryonic day E13.5, E15.5 and E17.5. Sortilin show marked central expression in developing costae (a-c), as well as in the chondral primordium and later ossifying vertebrae (d-f). Sortilin expression is absent from brown adipose tissue until E17.5 where it is prominent (g,h). Green channel is sortilin and blue channel is Hoechst nuclear stain. Scale bars = 50 μm. Ba, brown adipose tissue; Co, costae; Ve, vertebrae