Fig. 8.

The hys mutant displays respiratory and flight defects. (A) CO₂ production was measured for white¹¹¹⁸, the WT strain Canton-S (C-S), and the mutant hys after 7 days under normoxia. The mutant hys displays a 31% decrease in metabolic rate (P < 0.001). (B) Flight ability was measured in a simple flight assay, as described (40). In brief, control (C-S) flies were dropped into a 500-ml graduated cylinder with its internal walls coated with paraffin oil. Normal flies quickly initiate horizontal flight, striking the wall close to the entry level, whereas poor fliers land at lower levels or at the bottom of the cylinder. (C) The hys mutants (2 days old) perform poorly.