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. 2016 Mar 10;17:217. doi: 10.1186/s12864-016-2397-8

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© Bar et al. 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License(http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Preparation of Southern bluefin tuna gonad samples for RNA extraction. a – Gonad dissection (ovaries from females and testes from males); b – Enzymatic digestion of the crude gonads to break down the tissue to individual cells; c – Percoll gradient enrichment of ASG from the testes cells; d – Filtration through a 30 μm pore size filter to enrich oogonia cells from the ovary cells; e – RNA extraction from each of the 4 groups (testes/ovaries, both crude/enriched)