Table 1.
List of primers used in this study
| Name | 5´–3´ Sequence |
|---|---|
| PlacSynth_SacI_EcoRI_F | GATGACGAGCTC GAATTCTGGTCTTTATTCTTCAA |
| M13_R_NheI | CGACGAGCTAGCAGCCAGGAAACAGCTATGACC |
| mCherry_RBS_XbaI | GCTGCTTCTAGAAAGGAGGAAATTATACATGTTATCAAAGGGTGAAGAAG |
| mCherry_R_BamHI | CGTCGTGGATCCTTATCACTTGTATAATTCATCCATACC |
| Tldh_amp_R_PstI | CTGCTGCTGCAGAAAAAGATTAAAAAAGCCGCTGC |
| mCherry_seq_R | TGGACGACCTTCACCTTCAC |
| mCherry_seq_F | AACGTATGTACCCAGAAGATG |
| CAT_seq2_back | TACATCATTCTGTTTGTGATGG |
| B_mega_XylOP_out_F | AACATATAAACAGCCAGTTGCC |
| B_mega_XylOP_R(SpeI, ScaI, BamHI) | GTAGTAGGATCC AGT ACTAGTTTCCCCCTTTGATTTAAGTG |
| mCherry_w/o_RBS_XbaI | CGTCGTTCTAGAATGTTATCAAAGGGTGAAGAAGATAAC |
| p256_miniori_for | CATCATAAGCTTCCCGCACGCATAGCGGTGC |
| B_mega_XylOP_F_MfeI, KpnI | GTAGTACAATTG GGTACCAAGGTGAGGGTGGAGACAG |
| Bmega_XylR_newRBS_XbaI_Phos_R | GTATAATTTCCTCCTTTCTAGAAGTGAACAAGTTTATCCAT |
| mCherry_Phos_F | ATGTTATCAAAGGGTGAAGAAG |
| B_mega_XylOP_seq_F | CAATTCCGATATTAATACTGATG |
| B_mega_XylOP_seq_R | CTAGTCGGAATAGGAATTTGTG |
| LacI_Lplant_F_SacI | AGCAGCGAGCTCCCTAATAGAACTGCGGTGGTC |
| LacI_Lplant_R_XbaI | AGCAGCTCTAGAAACTTAATTACGCTTTAACAGC |
| lacR_Gal_seq_R | AATTGAAGTGATGCGGGTCTG |
| lacR_Gal_seq_F | AATTGCGCCAGCTAACACCC |
| T7_RNAP_Lp_RBS | CAGCAGTCTAGATCCTAAAGGAGG |
| T7_RNAP_Lp_Term_R_SalI | CAGCAGGTCGACTTGATATACAAAAAAGG |
| M13_2_F | TTGTAAAACGACGGCCAGTG |
| T7-Promoter_SacI | GCTGCTGAGCTCAGATCGATCTCGATCCCGCG |
| T7-Terminator_SalI | GCTGCTGTCGACTCCGGATATAGTTCCTCCTTTC |
| ery_back_KasI | CATCATGGCGCCTCCGATTGCAGTATAAATTTAACG |
| oripE194_seq_back | AATCAAATCGGTATAAATCTGAC |
| Ery_F_NheI | CATCATGCTAGCTCCGATTGCAGTATAAATTTAACG |
| Pempty_SacI_R | TAGTAGTCTA GA GCTCGAATTCACTGGCCGTCG |
| mCherry_RBS_SacI_F | GCTGCTGAGCTCAAGGAGGAAATTATACATGTTATCAAAGGGTGAAGAAG |
Restriction sites are underlined and highlighted in bold or italics